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The Effects of Methylated Cyclodextrins on the Transphosphorylation among AMP, ADP and ATP in the Presence of Escherichia Coli The Effects of Methylated Cyclodextrins on the Transphosphorylation among AMP, ADP and ATP in the Presence of Escherichia Coli 高橋, 圭子 タカハシ, ケイコ SONE, Hironobu SHIMIZU, Toshihiko 服部, 憲治郎 ハットリ, ケンジロウ TAKAHASHI, Keiko SONE, Hironobu SHIMIZU, Toshihiko HATTORI, Kenjiro Transphosphorylation, 2ADP⇄AMP+ATP, occurred in the presence of intact Escherichia coli (E. coli. strain K-12) in neutral phosphate buffer solution, because adenylate kinase existed in both cytoplasma and periplasma of E. coli. Cyclodextrin(CD)s accelerated the transphosphorylation. The reaction rate depended on the concentration of CD and the kind of CD. Methylated CDs were more effective than parent CD, especially, hiptakis-(2,6-di-O-methyl)-β-CD(DMCD) was most effective. Addition of CDs makes neither change of E. coli concentration nor the leak of adenylate kinase from periplasma of E. coli. 5 KJ00001511967 論文 Article 1991 eng departmental bulletin paper https://kougei.repo.nii.ac.jp/records/192 AN00159741 03876055 東京工芸大学工学部紀要 The Academic Reports, the Faculty of Engineering, Tokyo Polytechnic University 14 1 22 26 https://kougei.repo.nii.ac.jp/record/192/files/KJ00001511967.pdf application/pdf 332.0 kB 2017-04-21